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. 2020 May 6;17:146. doi: 10.1186/s12974-020-01811-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Microglia do not influence the number of early intermediate developing astrocyte populations in the tuberal hypothalamus. a Representation of Olig2, Aldh1L1, Sox9, and S100β immunostaining from control and PLX5622 treated P2 brains. Third ventricle outlined with dotted white line. b Aldh1L1+/Olig2+ cell counts (P = 0.9939) and c S100β+/Sox9+ cell counts (P = 0.5662) in the tuberal hypothalamus from control and PLX5622 treated P2 brains. Samples: Counts combined from three brain slices in n = 5, 6 in Aldh1L1+/Olig2+ counts and n = 4, 4 for S100β+/Sox9+ counts for control and PLX5622 brains, respectively. Graphs: Box and Whisker plots with middle line representing median, cross representing mean, box extending from the 25th to 75th percentiles, and whiskers at max and min values. Statistics: Student’s t test