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. 2020 May 6;20:109. doi: 10.1186/s12866-020-01798-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Growth curves of REL606 expressing hpaB variants in defined medium with 4HPA (a) or 3HPA (b) as sole carbon source. E. coli REL606 cells harboring expression constructs BL21(DE3)-derived hpaB [pHCE-IIB-HpaB(R379), ]; REL606-derived hpaB [pHCE-IIB-HpaB(C379), ]; mutant hpaBs [(pHCE-IIB-HpaB(G379), ]; and pHCE-IIB-HpaB(S379), ]. REL606 harboring the control plasmid (pHCE-IIB, ) was used as control. The culture medium comprised defined medium supplemented with 0.76 g/L (5 mM) of 3HPA or 4HPA. Error bars denote standard deviation of the mean from three independent cultures

Inline graphic — Growth curves of REL606 expressing hpaB variants in defined medium with 4HPA (a) or 3HPA (b) as sole carbon source. E. coli REL606 cells harboring expression constructs BL21(DE3)-derived hpaB [pHCE-IIB-HpaB(R379), ]; REL606-derived hpaB [pHCE-IIB-HpaB(C379), ]; mutant hpaBs [(pHCE-IIB-HpaB(G379), ]; and pHCE-IIB-HpaB(S379), ]. REL606 harboring the control plasmid (pHCE-IIB, ) was used as control. The culture medium comprised defined medium supplemented with 0.76 g/L (5 mM) of 3HPA or 4HPA. Error bars denote standard deviation of the mean from three independent cultures