Table 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Description | Source |
|---|---|---|
| Strains | ||
| E. coli REL606 | E. coli str. B F−, tsx-467(Am), araA230, lon−, rpsL227(strR), hsdR−, [mal+](LamS) | Richard E. Lenski |
| E. coli BL21(DE3) | E. coli str. B F−ompT gal dcm lon hsdSB(rB−mB−) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) | F. William Studier |
| Plasmids | ||
| pHCE-IIB | Expression vector carrying the ampicillin resistance gene and a high-level constitutive expression promoter (HCE) | [31] |
| pHCE-IIB-HpaB(R379) | pHCE-IIB carrying hpaB with arginine at the 379th position [equivalent to hpaB of E. coli BL21(DE3)] | This study |
| pHCE-IIB-HpaB(C379) | pHCE-IIB carrying hpaB with cysteine at the 379th position (equivalent to hpaB of E. coli REL606) | This study |
| pHCE-IIB-HpaB(G379) | pHCE-IIB carrying hpaB with glycine at the 379th position (R379G substitution) | This study |
| pHCE-IIB-HpaB(S379) | pHCE-IIB carrying hpaB with serine at the 379th position (R379S substitution) | This study |