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. 2020 May 5;20:190. doi: 10.1186/s12870-020-02370-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Effects of knocking out and overexpression of gma-MiR398c composite soybean plants on drought resistance. a Performance of soybean with K599, OE-miR398c, KO-1 and KO-2 transgenic roots after drought stress. b Enlargement of the leaves from the soybean plants. c Relative electrolyte leakage in leaves from the soybean with transgenic hairy roots compared to the normal K599. (d) O₂^.− production rate in plant leaves. Control K599 represents hairy roots transformed with the empty vector; OE indicates transgenic hairy roots overexpressing gma-MIR398c; KO indicates transgenic hairy roots harboring the pCas9-U6-sgRNAs plasmid. Data are means of ten biological replicates ± SD (10 plants per replicate). Asterisks indicate significant difference applying Student’s t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001)