Table 1.
Monoclonal antibodies used in IHC staining
| Antibody | Clone | Concentration | Target cells | Company |
|---|---|---|---|---|
| ER | SP1 | 1:400 | Epithelial cells | Thermo Scientific, Waltham, USA |
| CD4 | 4B12 | 1:100 | CD4+ T-cells | Novocastra, Newcastle Upon Tyne, UK |
| CD8 | C8/144B | 1:50 | CD8+ T-cells | DAKO, Agilent Technologies, Santa Clara, CA, USA |
| CD20 | L26 | 1:1000 | B-cells, neoplasms of B-cells | DAKO |
| CD68 | PG-M1 | 1:400 | B-cells, macrophages, histiocytes, dendritic cells, NK cells | DAKO |
| CD138 | B-A38 | 1:50 | Plasma cells and some epithelial cells | AbD Serotec (BioRad), Kidlington, UK |
| PAX5 | 24 | 1:100 | B-cells | BD Biosciences, San Diego, USA |
| Actin | 1A4 | 1:300 | Fibroblasts | DAKO |
Antibody, clone, concentrations used, Target cells and company of the antibodies used in IHC staining