Table 2.

Precision, accuracy, matrix effect, and recovery of hirsutine and hirsuteine in mouse tissue (n = 6).

Compound	Tissue	Concentration (ng/mL)	Precision (%)		Accuracy(%)		Recovery	Matrix effect
			Intraday	Interday	Intraday	Interday
Hirsutine	Kidney	4	8.4	7.4	96.5	103.3	76.3	91.7
		450	3.0	5.3	98.6	98.2	73.0	86.2
		4500	8.2	4.5	101.9	96.8	77.6	86.5
	Liver	4	12.9	14.5	97.1	102.9	85.9	94.9
		450	10.2	10.6	109.1	90.9	85.9	91.0
		4500	4.0	8.0	92.8	107.2	77.8	94.0
	Heart	4	12.4	13.3	90.8	89.4	85.9	95.2
		450	7.8	5.5	104.7	104.2	79.3	95.1
		4500	2.6	9.9	98.2	101.8	89.3	96.1
	Spleen	4	7.0	11.2	105.0	96.1	89.3	98.4
		450	8.7	13.2	96.1	96.5	77.3	91.1
		4500	2.6	4.2	101.0	103.2	80.2	97.1
	Brain	4	5.4	3.5	98.2	103.3	82.6	93.7
		450	3.7	2.8	104.3	102.5	84.5	99.2
		4500	2.9	2.9	97.0	98.8	93.7	98.2
	Lung	4	8.5	8.5	100.2	110.0	91.8	96.5
		450	3.6	5.2	106.9	94.1	92.8	98.6
		4500	3.3	4.5	94.6	108.1	95.5	101.9
Hirsuteine	Kidney	4	9.0	7.8	96.2	103.1	76.5	92.5
		450	1.7	4.7	98.6	98.4	86.9	87.3
		4500	6.7	4.8	102.9	98.5	81.4	90.8
	Liver	4	11.6	12.2	96.5	113.6	86.9	102.5
		450	13.5	11.0	110.1	93.3	87.4	104.7
		4500	7.6	11.5	100.8	103.7	84.7	99.6
	Heart	4	9.6	13.0	93.8	88.8	83.6	103.4
		450	8.7	8.7	99.9	104.7	83.0	101.6
		4500	8.7	7.4	101.8	99.5	87.4	103.8
	Spleen	4	10.5	12.9	94.1	93.6	83.5	97.6
		450	7.9	8.7	107.7	95.3	73.9	95.4
		4500	8.6	3.2	99.3	103.6	85.9	103.9
	Brain	4	12.8	10.6	107.0	109.9	88.5	102.2
		450	8.3	8.3	103.8	98.6	88.3	95.6
		4500	4.2	7.2	98.8	98.3	85.6	104.5
	Lung	4	11.2	13.8	104.5	94.3	83.4	97.3
		450	6.2	7.3	96.9	104.8	80.2	103.0
		4500	3.4	8.3	99.6	98.3	86.9	95.9