Table 2.
. Analytical sensitivity for pool plasma samples (96 samples/pool) *
Copies of external quantification standard RNA spiked in minipools | Number positive/ number tested | Percent positive |
---|---|---|
80 | 10/10 | 100 |
60 | 10/10 | 100 |
40 | 10/10 | 100 |
20 | 10/10 | 100 |
10 | 9/10 | 90 |
5 | 8/10 | 80 |
0 | 0/10 | 0 |
External quantification standard was extracted from 9.6 mL of 96 pooled donor samples after centrifugation. Five microliters of 75‐µL nucleic acid extract was analyzed. The 95 percent detection limit was 93 copies/mL; the 50 percent detection limit was 43 copies/mL.