Table 1. Effects of BK channel blockers on NMJ function in physiological solution.

Toxin (company)	Number of mice (number of NMJs)	Results (before vs. after toxin)	Nested ANOVA (P value)
Iberiotoxin (Sigma-Aldrich), 400 nM	4 (43 before, 51 after)	Quantal content, 58.8 ± 2.6 vs. 60.0 ± 2.4; mFreq, 2.8 ± 0.7 vs. 4.0 ± 0.6	Quantal content, 0.825; mFreq, 0.224
Iberiotoxin (Cayman), 400 nM	3 (37 before, 50 after)	Quantal content, 41.5 ± 2.7 vs. 41.7 ± 2.2; mFreq, 3.58 ± 0.6 vs. 4.7 ± 0.5	Quantal content, 0.959; mFreq, 0.121
Charybdotoxin (Alomone), 400 nM	6 (70 before, 96 after)	Quantal content, 57.8 ± 1.8 vs. 55.3 ± 1.5; mFreq, 4.8 ± 0.7 vs. 4.8 ± 0.6	Quantal content, 0.287; mFreq, 0.926
Charybdotoxin (Cayman), 400 nM	3 (45 before, 56 after)	Quantal content, 57.9 ± 2.7 vs. 56.2 ± 2.4; mFreq, 6.1 ± 0.8 vs. 7.8 ± 0.7	Quantal content, 0.628; mFreq, 0.119
Paxilline (Sigma-Aldrich), 10 µM	5 (55 before, 63 after)	Quantal content, 48.6 ± 1.5 vs. 44.7 ± 1.7; mFreq, 4.6 ± 0.8 vs. 5.0 ± 0.9	Quantal content, 0.079; mFreq, 0.750

Results are shown as mean ± SEM. mFreq, frequency of mEPCs in the absence of nerve stimulation.