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. 2020 Apr 7;152(5):e201912492. doi: 10.1085/jgp.201912492

Figure 4.

Figure 4.

Acute application of dopamine but not chlorpromazine inhibits CaV2.2 currents in transfected HEK293T cells. (A) Representative traces of CaV2.2 currents from HEK293T cells transfected with CaV2.2, CaVα2δ1, CaVβ3, and D1R (11 or 23 ng cDNA) before and after acute application of dopamine (+DA; 10 µM) or chlorpromazine (+CPZ; 10 µM). (B) Concentration-response curve for dopamine (+DA) and chlorpromazine (+CPZ; n = 3, 1 µM and n = 4, 10 µM) effect on CaV2.2 currents (ICav2.2). Data on dopamine effect were fitted with Hill equations for each D1R cDNA amount used (EC50 = 2.023 µM for 11 ng, total n = 42, r2 = 0.77 and EC50 = 1.180 µM for 23 ng of D1R, total n = 61, r2= 0.86). EC50, half-maximal effective concentration. Data were expressed as mean ± SEM.