Figure S1.
Deletion of nvNUDT9-H coding sequence results in channels indistinguishable from nvTRPM2(1208STOP). (A) Macroscopic current activated by cytosolic exposure to increasing concentrations of ADPR (gray bars) plus 125 µM Ca2+ (black bar), recorded from an inside-out patch excised from a Xenopus oocyte preinjected with cRNA encoding nvTRPM2(1208CUT). (B) Steady-state currents in the presence of test [ADPR], normalized to the mean of the currents during bracketing exposures of the same patch to 320 µM ADPR, plotted as a function of [ADPR] (orange symbols). Data represent mean ± SEM from 5 to 12 independent experiments. Fits to the Hill equation (colored lines) yielded fit parameters of EC50 = 4.6 ± 0.3 µM and nH = 1.7 ± 0.2. Red (1208STOP) and dark blue (full-length) symbols replot data from Fig. 1 D for comparison.