Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 13;152(5):e201912491. doi: 10.1085/jgp.201912491

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Kawanabe et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — eVSP CiDr-VSP^mChe L223F. (A) Representative Kir2.1 current traces recorded from HEK293T cells coexpressing Dr-VSP^mChe WT, Dr-VSP^mChe L223F, CiDr-VSP^mChe WT, CiDr-VSP^mChe L223F (eVSP), CiDr-VSP^mChe L223F/C302S (eVSP with activity-null mutation: LF/CS), and Kir2.1 alone (−VSP). Holding potential was set to −60 mV and depolarizing step for activating VSP was repeatedly applied (21 times) to 100 mV for 300 ms (the protocol shown at the top). All 21 traces were superimposed in each panel. (B) Time-dependent changes in the normalized amplitudes of Kir2.1 currents in cells coexpressing Dr-VSP^mChe WT (unfilled gray circles), Dr-VSP^mChe L223F (unfilled black squares), CiDr-VSP^mChe WT (filled gray circles), CiDr-VSP^mChe L223F (filled black squares), CiDr-VSP^mChe L223F/C302S (LF/CS, unfilled gray triangles), or Kir2.1 alone (−VSP, unfilled black triangles) with repeated depolarizing pulses to 100 mV. Amplitudes of Kir2.1 currents were measured at the end of the hyperpolarizing test pulses (−120 mV, 50 ms; arrows in A). Values were normalized by the current amplitude during the first test pulse. Symbols depict the mean ± SD from 6 to 11 cells. (C) Phosphatase activities of Dr-VSP^mChe WT, Dr-VSP^mChe L223F, CiDr-VSP^mChe WT, CiDr-VSP^mChe L223F (eVSP), CiDr-VSP^mChe L223F/C302S (LF/CS), and Kir2.1 alone (−VSP). Rate constants for the declines in normalized Kir2.1 currents with single exponential fitting are shown as phosphatase activities. The values of the mean ± SD and individual plots are shown. Two cells expressing Dr-VSP^mChe WT showed no phosphatase activity and were excluded from these data (*). Values in parentheses indicate the numbers of cells. Upper bars show P values from a two-tailed Student’s t test. (D) Phosphatase activities estimated by different depolarizing conditions. White, light gray, dark gray, and dim gray bars show Dr-VSP^mCheWT, Dr-VSP^mChe L223F, CiDr-VSP^mChe WT, and CiDr-VSP^mChe L223F, respectively. Horizontal axis shows the depolarizing voltage condition: 0, 25, 50, 75, and 100 mV. The data in 100 mV are the same data as those shown in C.