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. Author manuscript; available in PMC: 2021 Mar 23.
Published in final edited form as: Dev Cell. 2020 Mar 23;52(6):699–713.e11. doi: 10.1016/j.devcel.2020.02.011

Figure 6. New pS22-Lamin A/C-binding sites emerged in progeria are associated with up-regulation of genes relevant to progeria phenotypes.

Figure 6.

(A) pS22-Lamin A/C ChIP-seq profiles (derived from 2 biological replicates) at representative gained and lost pS22-Lamin A/C-binding sites. Normal, GM07492 fibroblast. Progeria, AG11498 fibroblast. Horizontal bar, 2 kb. See also Fig. S6.

(B) pS22-Lamin A/C ChIP-seq FE scores at gained and lost pS22-Lamin A/C-binding sites in the normal GM07492 and progeria AG11498 fibroblasts.

(C) Chromatin states of gained and lost pS22-Lamin A/C-binding sites. Fisher’s exact test assesses association between being called as gained or lost and being affiliated with each state.

(D) Log2 difference of c-Jun ChIP-seq FE scores between normal (GM07492) and progeria (AG11498) fibroblasts (derived from 2 biological replicates). Red dashed line, log2 difference equals 0 for reference.

(E) Same as (D), but H3K27ac ChIP-seq scores are analyzed.

(F) Fraction of progeria-up and progeria-down genes with gained and/or lost pS22-Lamin A/C-binding sites (within gene body or 100 kb upstream). P, Fisher’s exact test P value for association between being differentially expressed and being affiliated with gained/lost pS22-Lamin A/C-binding sites.

(G) DisGeNet-curated disease terms over-represented among progeria-up genes linked to gained pS22-Lamin A/C-binding sites, or those not linked to gained pS22-Lamin A/C-binding sites, or all progeria-up genes.