Skip to main content
. 2020 Mar 9;217(5):e20190888. doi: 10.1084/jem.20190888

Figure 2.

Figure 2.

Trib1 deficiency promotes elevated CD4 effector cell expansion and function in response to chronic infection. (A and B) Representative flow plots (A) and summary of the frequency of CD4 effector T cells (B; CD44+CD62L−, gated on CD4+) from peripheral blood (PB) at day 8 after clone 13 infection. (C and D) Representative flow plots (C) and summary of cytokine production in splenic CD4 T cells harvested from mice at day 12 p.i. with clone 13 and stimulated ex vivo with PMA and ionomycin for 5 h (D; gated on CD4+). (E and F) Representative flow plots (E) and summary of IL-2 cytokine production in splenic CD4 T cells harvested from mice at day 12 p.i. and stimulated as in C and D (F). (G) Relative Il-2 expression measured by qPCR from naive CD4 T cells cultured for 24 h (left) or IL-2 secretion measured by ELISA (right) from CD4 T cells cultured for the indicated time points ± anti-CD3/CD28 stimulation. (H–J) Representative flow plots (H) and summary of the frequency of Foxp3+ (I) and Foxp3+CD25+ T reg cells in the spleen at day 30 after clone 13 infection (J). (K) Representative histogram (left) and summary (right) of the MFI of CD44 expression on splenic T reg cells at day 30 after clone 13 infection. (L) Representative histogram (left) and summary (right) of the MFI of CTLA4 expression on splenic T reg cells at day 30 after clone 13 infection. (M and N) Representative flow plots (M) and summary of the frequency of PD1+ T reg cells in the spleen of mice at day 30 p.i. (N). Data in A and B are representative of four independent experiments each with 6–10 mice per genotype. Data in C–F are representative of two independent experiments each with 7–10 mice per genotype. Data in G are representative of two (left) and more than five (right) independent experiments. Data in I are representative of two independent experiments each with 6–10 mice per genotype. Data in J–N represent an experiment with 6–8 mice per genotype. CT values normalized to 18s ribosomal RNA. Error bars are mean ± SD. **, P < 0.01; ****, P < 0.0001 by unpaired Student’s t test. Control: CD4-cre+ Trib1+/+; Trib1 cKO: CD4-cre+ Trib1F/F. n.s., not significant.