Skip to main content
. 2020 Mar 9;217(5):e20190888. doi: 10.1084/jem.20190888

Figure 3.

Figure 3.

Trib1 deficiency promotes CD8 KLRG1+ effector-like cell expansion and CD8 function in response to chronic infection. (A and B) Representative flow plots (A) and summary of the frequency of CD8 effector-like T cells (KLRG1+CD127−, gated on CD8+CD44+) from peripheral blood (PB) at day 8 after clone 13 infection (B). (C and D) Representative flow plots (C) and summary of cytokine production in splenic CD8 T cells harvested from mice at day 12 p.i. with clone 13 and stimulated ex vivo with PMA and ionomycin for 5 h (D; gated on CD8+). (E and F) Representative flow plots (E) and summary of CD107a staining on splenic CD8 T cells harvested from mice at day 12 p.i. and stimulated ex vivo as in C and D (F). Data in A and B are representative of four independent experiments each with 6–10 mice per genotype. Data in C–F are representative of two independent experiments each with 7–10 mice per genotype. Error bars are mean ± SD. ***, P < 0.001; ****, P < 0.0001 by unpaired Student’s t test. Control: CD4-cre+ Trib1+/+; Trib1 cKO: CD4-cre+ Trib1F/F.