Figure S2.

Trib1 cKO T_EX subset phenotype is established early in chronic infection and before differences in viral burden between control and Trib1 cKO mice. (A) Viral titers 8, 15, 22, and 30 d p.i. in sera from mice infected with clone 13 LCMV. Data represent one independent experiment with 9 or 10 mice per genotype. (B and C) Representative flow plots (B) and summary of the frequency of Ly108/GranzymeB^hi/lo CD8 T cells (gated on CD8⁺) from the spleens of mice at day 12 p.i. (C). (D and E) Summary of T-bet expression (measured by MFI) in splenic Ly108^loGranzymeB^hi (D) or Ly108^hiGranzymeB^lo CD8 T cells at day 12 p.i. (E). Day 30 titers are representative of three independent experiments with a second experimental cohort (of the three) represented in Fig. 1 K. Data in B and C are representative of two independent experiments each with 6-10 mice per genotype. Data in D and E represent an experiment with seven or eight mice per genotype. Error bars are ± SEM in A and ± SD in B–E. *, P < 0.05; **, P < 0.01; ***, P < 0.001 by unpaired Student’s t test. Control: CD4-cre⁺Trib1^+/+; Trib1 cKO: CD4-cre⁺Trib1^F/F.