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. 2020 Mar 6;217(5):e20192282. doi: 10.1084/jem.20192282

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© 2020 Shi et al.

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Figure S2. — Type I IFN could be up-regulated in bone marrow–derived DCs cocultured with tumor cells and Bifidobacterium after CD47 blockade. Related to Fig. 3. qPCR of Ifnb mRNA levels in bone marrow–derived DCs. Bone marrow–derived DCs (2 × 10⁶) were cocultured with MC38 cells (2 × 10⁶) with 10 µg/ml anti-CD47 or 10 µg/ml rat IgG in the presence or absence of 2 × 10⁶ Bifidobacterium for 8 h. DCs were then sorted out for Ifnb quantification by qPCR. Presented as mean ± SEM. *, P < 0.05 (nonpaired Student’s t test).