Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 17;217(5):e20191340. doi: 10.1084/jem.20191340

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Zhan et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — The MBD3/NuRD complex promotes H3K27 deacetylation on STAT1 promoter to inhibit STAT1 expression in GSCs. (A) ChIP analyses on STAT1 promoter. Assays were performed with the H3K27ac (left, n = 3) and H3K27me3 (right, n = 3) antibodies, and immunoprecipitates were subjected to qPCR analyses. (B) ChIP analysis with MBD3 antibody showing the enrichment of MBD3 at the promoter of STAT1 (around primer 6) in T4121GSCs and T387GSCs. Schematic showing the ChIP primer location with respect to the TSS of the STAT1 promoter (top). (C and D) ChIP analysis on the promoter of STAT1 in T4121GSCs (n = 3) and T387GSCs (n = 3) expressing shNT or two independent shMBD3s. Assays were performed with the indicated antibodies, and immunoprecipitates were subjected to qPCR analyses (primer 6). (E) IB analysis of STAT1, STAT3, and MBD3 in T387GSCs and T4121GSCs expressing shNT or two independent shMBD3s. (F) ChIP analyses on STAT1 promoter in GSCs and matched NSTCs. Assays were performed with the HDAC1 (left, n = 3) and CHD4 (right, n = 3) antibodies, and immunoprecipitates were subjected to qPCR analyses (primer 6). (G) Proposed model for MBD3/NuRD-mediated regulation of STAT1 transcription. In GSCs, MBD3 is highly expressed and binds to STAT1 promoter, recruits the NuRD complex (including CHD4 and HDAC1) to suppress STAT1 expression by H3K27 deacetylation. Loss of MBD3 disassembles the NuRD complex, increases H3K27 acetylation, and promotes STAT1 transcription. Data are represented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001, as assayed by unpaired Student’s t test or Welch’s t test.