Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 17;217(5):e20191340. doi: 10.1084/jem.20191340

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Zhan et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — MBD3 is preferentially expressed in GSCs. (A) IB analysis of MBD3, MBD2, SOX2, and GFAP in GSCs and matched NSTCs derived from five human GBM tumors. (B) IB analysis of MBD3, MBD2, SOX2, and GFAP during GSC differentiation. (C) IB analysis of MBD3, STAT1, SOX2, and Olig2 in GSCs and NHAs. (D) Co-IF staining of MBD3 (green) and SOX2/Olig2 (red) in human GBM specimens. Nuclei were counterstained with Hoechst (blue). (E) IHC staining of MBD3 in brain tumor tissue microarray. Section was counterstained with hematoxylin (left). Box plot of histoscore of MBD3 (right). Normal brain tissue (n = 5), low-grade gliomas (I–II, n = 15), and high-grade gliomas (III–IV, n = 39). One-way ANOVA; *, P < 0.05. (F) IHC staining of MBD3 (left) and STAT1 (right) in serial sections of human GBM specimens. Sections were counterstained with hematoxylin. (G) Co-IF staining of STAT1 (green) and MBD3 (red) in human GBM specimen and mouse GBM orthotopic xenograft. Nuclei were counterstained with Hoechst (blue).