Figure 2.

Prolonged exposure to myelin promotes an inflammatory phagocyte phenotype. (A–C) mRNA expression of inflammatory factors in LPS-stimulated human MDMs (n = 7 wells), mouse BMDMs (n = 5 wells), and mouse microglia (n = 6 wells) treated with myelin for 24 or 72 h, or left untreated (dotted line, Ctrl). (D–L) Representative immunofluorescence images and quantification (MFI of CCR7, CD32, and IL-1B in phagocytes, and number of phagocytes expressing CCR7, CD32, and IL-1B) of an active MS lesion stained with CCR7/CD68, CD32/IBA1, and IL-1B/IBA1 (n = 3 lesions from three different MS patients). Scale bars, 500 µm (overview); 50 µm (inset). Results are pooled from or representative of two (B and C) or three (D–L) independent experiments. Human MDM cultures from seven donors were used (A). All data are represented as mean ± SEM. *, P < 0.05, **, P < 0.01, and ***, P < 0.001, unpaired Student’s t test (A–C), one-way ANOVA (G–L).