Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 18;217(5):e20182218. doi: 10.1084/jem.20182218

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Zhang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Analysis of iNKT differentiation in USP22 cKO mice. (A–C) TCRβ and CD1d tetramer-positive iNKT cells in WT and USP22 cKO thymocytes were gated, and their expression of PLZF and ROR-γT (A), or T-bet (B), or GATA3 (C) was determined by intracellular staining and flow cytometry. (D and E) The average percentages (D) and absolute numbers (E) of iNKT1, iNKT2, and iNKT17 from six pairs of WT and USP22 cKO mice are shown. (F and G) The lineage-specific cytokine production in total splenic iNKT cells was determined by intracellular staining. Representative images (F) and data from six pairs of mice (G) are shown. Each symbol (D, E, and G) represents an individual mouse. Error bars represent mean ± SD. Student’s t test was used for statistical analysis. **, P < 0.01; ***, P < 0.001. In A–G, data are pooled from six independent experiments.