Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 18;217(5):e20182218. doi: 10.1084/jem.20182218

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Zhang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — Vα14-Jα18 TCR transgenic expression restores iNKT cell development in USP22 cKO mice. (A–C) iNKT cells in the thymus (Thy), spleen (Spl), and liver from Vα14-Jα18/USP22^+/+ and Vα14-Jα18/USP22^−/− mice were determined by flow cytometry. Representative images (A), the percentages (B), and absolute numbers (C) of iNKT cells from seven pairs of mice are shown. (D and E) The developmental stages were analyzed as in Fig. 3. Representative images (D) and data from four pairs of mice (E) are shown. (F and G) The levels of T-bet, IL-2Rβ, PLZF, and EGR2 in thymic iNKT cells were analyzed. Representative images (F) and the average MFI (G) from four pairs of mice are shown. (H) A proposed model for USP22-mediated histone H2A deubiquitination through MED1 interaction to regulate genes critical for iNKT development. Each symbol (B, C, E, and G) represents an individual mouse. Error bars represent mean ± SD. Student’s t test was used for statistical analysis. *, P < 0.05; **, P < 0.01. Data are representative of five experiments (A–C) or three experiments (D–G). TF, transcriptional factor.