Figure 3.

Histology of melorheostotic bones. (A) Light microscopy images of cortical bone from patients Melo-11 and Melo-12 (upper row). Goldner’s trichrome staining is viewed under polarized light (lower row). Green is mineralized bone. Note the vascular channels with concentric arranged bone lamellae (asterisks) in Melo-11 unaffected and affected bone. The arrows point toward parallel lamellar bone, which is only viewed in affected bone. In Melo-11, there is a mixture of concentric lamellar structure and parallel lamellar structure. In contrast, in Melo-12, the bone lamellae are perfectly parallel-oriented. Note that the vascular channels are rather small. Scale bars = 150 µm. (B) BMDD curves obtained by qBEI. The BMDD curves from affected bone from Melo-11 and Melo-12 shifted toward a higher degree of mineralization compared with unaffected bone from the same patient. In Melo-8 and Melo-17, the degree of mineralization in affected and unaffected bone is similar. Aff, affected; Unaff, unaffected.