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Analysis of purified αSTn-IgG4 TM by SDS-PAGE, Western Blot and size exclusion HPLC. The αSTn-IgG4 TM was purified from the supernatant of TM-producing 3T3 cells using protein A columns. The dialyzed TM was resolved by SDS-PAGE and stained with (a) Coomassie Brilliant Blue G250 or (b) analyzed by WB using αHis mAb for detection of the C-terminus His-tag. c Chromatogram obtained by size exclusion HPLC of 10 μg of purified TM
