Fig. 6.

In vivo killing capacity of redirected UniCAR T-cells in NOD-SCID mice using αSTn-IgG4 and αSTn TMs. Five groups composed of five NOD-SCID mice were established, from which mice injected with MDA-MB-231 STn⁺ Luc tumor cells alone; tumor cells mixed with αSTn-IgG4 TM or tumor cells mixed with UniCAR 28/ζ T-cells were used as negative controls. Mice co-injected with tumor cells, UniCAR 28/ζ T-cells and αSTn TM or αSTn-IgG4 TM were used as treatment groups. All injections were performed subcutaneously into the right hind flank. a Bioluminescence imaging of anesthetized mice was performed at day 0, 1 and 3. b Based on the luminescence imaging results obtained, a quantitative analysis was performed. The values were normalized to the initial measurement performed after injection on day 0 and represented as mean ± SEM. Statistical significance was determined using two-way ANOVA with Bonferroni multiple-comparison test with respect to the control group injected with MDA-MB-231 STn⁺ Luc and UniCAR 28/ζ T-cells (*p < 0.1; **p < 0.01; ***p < 0.001)