Fig. 8.

Downregulation of MET reverses the oncogenic phenotype induced by overexpression of circBFAR. a, b The upregulation of MET and p-Akt (Ser 473) in PANC-1 and BxPC-3 cells transfected with circBFAR was reversed by silencing MET, as detected using western blotting. GAPDH was used as a loading control. c, d CCK-8 experiments showing that transfection with circBFAR increased the proliferation ability of PANC-1 and BxPC-3 cells; however, this function was reversed after co-transfection with si-MET#1. e, f, g Wound healing, and Transwell invasion assays demonstrating that transfection with circBFAR increased the migration and invasion ability of PANC-1 and BxPC-3 cells; however, this function was reversed after co-transfection with si-MET#1. The images were photographed at 40X (e, f) or 100X (g) magnification. Scale bar =200 μm (e, f). Scale bar =100 μm (g). Statistical significance was assessed using two-tailed t-tests for two group comparison, and one-way ANOVA followed by Dunnett’s tests for multiple comparison. The error bars represent the standard deviations of three independent experiments. *P < 0.05, **P < 0.01