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. 2020 May 5;20:376. doi: 10.1186/s12885-020-06775-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — SNHG7 sponges miR-514a-5p in NPC. a Subcellular fractionation assay detected the distribution of SNHG7 in C666–1 and HNE1 cells. b starBase website screened out two miRNAs that possess the highest binding ability with SNHG7. c RNA pull down assay analyzed the interaction between SNHG7 and miR-668-3p (or miR-514a-5p). d The expression of miR-514a-5p in NPC cells and nasopharyngeal epithelial cell line was quantified by RT-qPCR. e The influences of SNHG7 silencing on miR-514a-5p expression were evaluated via RT-qPCR. f RIP assay proved that SNHG7 and miR-514a-5p coexisted in RISC. g The potential binding sites between SNHG7 and miR-514a-5p were displayed. h The overexpression efficiency of miR-514a-5p was quantified by RT-qPCR. i Luciferase activity assay demonstrated that SNHG7 could bind with miR-514a-5p. GAPDH or U6 was used as an internal control. Error bars represent the mean ± SD of at least three independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001