Table 1. Genome size estimates and assembly statistics.
| Species | Est. Genome Size (bp) | Total Scaffold Length (bp) | Scaffold NG50 (bp) | Longest Scaffold (bp) | Contig NG50 (bp) | Longest Contig (bp) | Total Gap Length (bp) |
|---|---|---|---|---|---|---|---|
| D. kanapiae | 155,490,160 | 152,203,088 | 389,587 | 2,274,126 | 301,459 | 2,274,126 | 205,953 |
| D. birchii | 169,148,727 | 156,593,892 | 211,718 | 1,501,252 | 164,580 | 1,183,551 | 81,207 |
| D. truncata | 190,688,284 | 167,897,087 | 95,737 | 830,117 | 73,889 | 827,712 | 375,696 |
| D. bocki | 155,095,574 | 151,202,254 | 78,068 | 785,450 | 65,314 | 785,450 | 69,231 |
| D. bunnanda | 181,250,127 | 151,823,105 | 76,713 | 1,142,480 | 65,403 | 1,127,760 | 152,423 |
| D. punjabiensis | 197,448,094 | 192,339,030 | 72,420 | 1,226,934 | 64,043 | 1,083,757 | 153,372 |
| D. jambulina | 179,468,675 | 163,991,206 | 71,637 | 873,064 | 61,348 | 756,687 | 61,365 |
| D. vulcana | 209,187,412 | 187,578,810 | 65,096 | 530,507 | 51,774 | 472,464 | 116,296 |
| D. seguyi | 206,814,592 | 178,856,532 | 63,109 | 891,413 | 54,123 | 891,413 | 233,653 |
| D. mayri | 223,398,425 | 167,807,061 | 62,249 | 2,219,437 | 43,922 | 1,355,909 | 364,662 |
| D. asahinai | 216,977,949 | 189,050,820 | 59,266 | 1,052,132 | 50,528 | 904,342 | 75,577 |
| D. serrata | 184,673,878 | 159,679,625 | 54,224 | 1,091,401 | 43,626 | 718,797 | 79,019 |
| D. lacteicornis | 203,475,870 | 182,681,050 | 53,799 | 1,044,495 | 44,105 | 766,914 | 60,537 |
| D. pectinifera | 220,219,034 | 149,209,000 | 52,632 | 528,734 | 41,478 | 467,725 | 58,142 |
| D. tani | 194,820,185 | 180,972,673 | 48,517 | 921,527 | 44,341 | 780,901 | 135,927 |
| D. rufa | 210,769,271 | 186,167,886 | 43,287 | 498,065 | 38,201 | 498,065 | 80,447 |
| D. watanabei | 182,199,997 | 196,825,890 | 40,952 | 1,045,963 | 36,818 | 656,929 | 135,921 |
| D. auraria | 220,036,088 | 197,420,731 | 38,365 | 491,046 | 35,679 | 491,046 | 130,216 |
| D. cf. bakoue | 219,308,053 | 187,248,584 | 28,924 | 1,045,797 | 27,520 | 598,596 | 58,713 |
| D. leontia | 162,918,854 | 164,601,511 | 26,461 | 331,217 | 23,897 | 301,031 | 91,716 |
| D. burlai | 198,129,694 | 175,666,184 | 24,417 | 628,960 | 23,536 | 628,960 | 153,793 |
| D. nikananu | 217,706,973 | 190,505,469 | 23,001 | 626,542 | 21,180 | 574,375 | 226,904 |
| D. triauraria | 217,036,792 | 197,369,186 | 17,513 | 590,840 | 16,493 | 576,941 | 156,570 |
Genome size estimates were calculated by SGA Preqc (Simpson 2014) based on the k-mer frequency spectrum of the unassembled reads. To calculate the scaffold NG50 (Earl et al. 2011; Bradnam et al. 2013), scaffold lengths were ordered from longest to shortest and then summed, starting with the longest scaffold. The NG50 was the scaffold length that brought the sum above 50% of the estimated genome size. Contig lengths were estimated by splitting scaffolds on every N, including single Ns. Species are listed in decreasing order of scaffold NG50.