Table 3. Effect estimates for generalized linear model (Poisson) using number of teeth in the ectopic/secondary sex comb as response variable. This model included only the HSS and LSS populations; WTC populations were excluded because they did not display ectopic sex combs, and because the purpose of this model was to test for a difference between the HSS and LSS treatments specifically, we considered the LV treatment on its own separately. The HSS and LSS treatments were constrained to have a common intercept in this model, but were allowed to have different slopes. The confidence intervals for the effect of generation in each treatment overlap.

	Estimate	Standard Error	P
Intercept	1.31	0.066	< 2.0 x 10−16
generation-HSS	−6.3 x 10−3	3.7 x 10−3	0.085
generation-LSS	−0.011	3.8 x 10−3	0.005