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. 2020 Mar 12;10(5):1553–1561. doi: 10.1534/g3.119.400972

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Copyright © 2020 McKinney et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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A) Individual PCA of RADseq samples using all loci from LG15. Examination of locus loadings show that Axis 1 is primarily driven by loci in set 1 (inversion loci) and axis 2 is primarily driven by loci in set 2 (sex-associated loci). Labels for each cluster of individuals denote the putative chromosome type of individuals within the cluster with respect to inversion and sex. B) Individual PCA of RADseq and GT-seq samples using loci successfully developed into GT-seq assays. Samples are color coded by phenotypic sex with gray individuals having unassigned sex.