Table 1. Number of samples initially sequenced and retained after quality filtering for RADseq and GT-seq datasets. Collections used to evaluate accuracy of the putative sex- determining region are marked with an asterisk *.

Region	Collection	Initial RADseq Samples	Retained RADseq Samples	Initial GT-seq Samples	Retained GT-seq Samples
Norton Sound	Eldorado River	48	48	83	82
Norton Sound	Fish River*	48	39	82	77
Norton Sound	Kwiniuk River*	0	0	82	74
Yukon River	Nulato River	48	38	83	80
Yukon River	Otter Creek	48	48	99	92
Yukon River	East Fork Andreafsky River	0	0	83	83
Kuskokwim River	Holokuk River	48	48	83	79
Kuksokwim River	Aniak River	0	0	82	79
Nushagak River	Kokwok River	48	46	83	79
Nushagak River	Mulchatna River*	0	0	82	73
		288	267	842	798