Table 2. Frequency of the chromosome inversion and sex-assignment accuracy by collection. Samples were assigned an inversion type (+/−) and sex based on PCA clustering. For collections with phenotypic sex data, phenotypes were compared to sex assigned through clustering to assess accuracy.

Region	Collection	Data Source	++	+-	–	Freq (-)	M cluster	F cluster	Sex Assignment Accuracy
Norton Sound	Eldorado River	RAD/GT-seq	96%	4%	0%	2%	50	80	NA
Norton Sound	Fish River	RAD/GT-seq	90%	10%	0%	6%	54	62	84%
Norton Sound	Kwiniuk River	GT-seq	92%	8%	0%	4%	30	44	89%
Yukon R.	Nulato River	RAD/GT-seq	73%	23%	4%	22%	65	53	NA
Yukon R.	Otter Creek	RAD/GT-seq	76%	22%	1%	16%	65	75	NA
Yukon R.	East Fork Andreafsky	GT-seq	83%	17%	0%	10%	37	46	NA
Kuskokwim R.	Holokuk River	RAD/GT-seq	89%	10%	1%	7%	54	73	NA
Kuskokwim R.	Aniak River	GT-seq	85%	15%	0%	9%	41	38	NA
Nushagak R.	Kokwok River	RAD/GT-seq	96%	4%	0%	2%	96	29	NA
Nushagak R.	Mulchatna River	GT-seq	90%	10%	0%	5%	37	36	99%