Table 1. The Cf-Ecp5 gene in S. pimpinellifolium G1.1161 is allelic or very tightly linked to the Cf-9 gene at MW. Test cross progeny were screened using a standard histological procedure for the presence or absence of GUS activity (GUS or no GUS) and for the presence or absence of Cf-Ecp5 (Necrotic or wild type respectively) based on the development of either a systemic necrosis phenotype following agro-infiltration with PVX:Ecp5 (crosses to Cf0 and LA0716) or a seedling lethal phenotype (crosses to Cf0:Ecp5).

Phenotype	Cf0 x (G1.1161 x M18)	LA0716 x (G1.1161 x M18)	Cf0:Ecp5 x (G1.1161 x M18)
GUS:wt	175	79	109
no GUS:N	201	62	108
GUS:N	0	0	0
no GUS:wt	0	0	0
Recombinant fraction	0%	0%	0%