Table 2. Unexpected suppression of Ecp5-related HR in crosses between different Cf-Ecp5-carrying accessions. Data from different populations following agro-infiltration with PVX:Ecp5. Phenotypes were recorded at 10-14 dpi. G1.1161 carries a Cf-Ecp5 gene on chromosome 1, Ont7522 and LA2852 carry a Cf-Ecp5 on chromosome 12, while Cf0 and LA0716 lack Cf-Ecp5. Population B851 lacked Cf-Ecp5.12p, while B852 and B853 carried it. In this study, LA2852 was usually crossed to Cf0 initially to form a bridged cross due to difficulties in direct crosses to other accessions. The cross, phenotypes (wild-type or Necrotic), expected ratios and p-values are shown (p-values were calculated in each cross based on the expected ratio if there was no suppression for dominant traits; ‘⊗↓’ = selfing; statistical significance was calculated based on chi-square tests: ‘***’=P ≤ 0.001; ‘**’=P ≤ 0.01; ‘*’=P ≤ 0.05).

Cross	wild-type	Necrotic	Expected Ratio (wt:N)	p-value(χ2)
(Ont7522 x (Cf0 x LA2852)) ⊗↓ (B851)	59	133	1:3	0.211
(Ont7522 x (Cf0 x LA2852)) ⊗↓ (B852)	42	362	0:1	0***
(Ont7522 x (Cf0 x LA2852)) ⊗↓ (B853)	41	364	0:1	0***
(G1.1161 x (Cf0 x LA2852)) ⊗↓	121	196	1:15	0***
(Ont7522 x LA0716) ⊗↓	74	81	1:3	3.159e-05***
(Cf0 x LA2852) x LA0716	124	52	1:1	8.836e-05***