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Multiple sequence alignments of 183 Sirt4 orthologs. (A) Histogram of the KA/KS substitution ratio for all of the pairwise alignments. (B) The average KA/KS from each species was plotted as a histogram. (C) Histogram plot of the KA/KS ratio from all of pairwise alignments with D. melanogaster (Ensembl Peptide ID: FBpp0070817).
