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Representative confocal immunohistochemical images (A) and group data (B) of TNF-α levels in the hypothalamic PVN of the following DM and respective control groups (n=5 rats each): male, ovariectomized (OVX), sham operated (SO), and OVX with E2 supplementation (OVX/E2). TNF-α images were analyzed by NIH ImageJ. Values are means ± SEM. *P < 0.05 vs. male control values. #P < 0.05 vs. DM male values.
