Representations of 3D-printed immunoarray: (A) 3D printed microfluidic array with chambers to hold sample, wash buffers, detection nanoparticles, and coreactant for ECL generation. Array is shown on the left without detection chip, and on the right bonded to a pyrolytic graphite sheet (PGS) microwell detection chip with reagent and sample chambers filled with dye solutions for visualization. (B) Representative disposable PGS chip with heat transferred microwells printed using hydrophobic toner ink. Inset illustrates a sandwich immunoassay on a single wall carbon nanotube forest (SWCNT) in one microwell. Reproduced from Kadimisetty, K.; Malla, S.; Bhalerao, K. S.; Mosa, I. M.; Bhakta, S.; Lee, N. H.; Rusling, J. F. Anal. Chem. 2018, 90, 7569–7577 (ref 47). Copyright 2018 American Chemical Society.