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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Am J Med Genet A. 2020 Mar 5;182(5):1104–1116. doi: 10.1002/ajmg.a.61531

Figure 5. The EDNRA p.Q381P variant disrupts G protein coupling.

Figure 5.

(a) Schematic of the BRET assay for G protein coupling. A Venus-mini G protein can associate with a wild type EDNRA-RLuc8 after addition of EDN1, resulting in BRET. (b) The maximum BRET response was quantified for four classes of mini G proteins with wildtype EDNRA-RLuc8 or EDNRA-RLuc8 p.Q381P. Maximum BRET response for all mini G proteins was lower in cells expressing EDNRA-RLuc8 p.Q381P compared to wildtype EDNRA, though the change for mGs12 was not statistically significant (p=0.0678). Assays were performed in triplicate at least three times. Error bars indicate SEM; two-tailed t-test ***p<0.005; ns, not significant.