Figure EV4. TLR4‐silenced MSCs reveal suppressed expression of TLR4 at transcriptional and protein levels.

1. MSCs were either treated with a scrambled control siRNA (Scr. siRNA) or with four different siRNAs targeting the TLR4 receptor (siRNA smart pool). Forty‐eight hours after transfection, TLR4‐silenced and non‐silenced MSCs were analyzed for RNA expression by qPCR. A representative experiment is depicted. Statistical analysis was performed using unpaired t‐test, and values are represented as mean ± SEM, three biological replicates.
2. Seventy‐two hours after transfection, TLR4‐silenced and non‐silenced MSCs were treated with 100 ng/ml LPS for 16 h and subjected to Western blot analysis for protein expression. Densitometric assessment shows the percentage of the change in TLR4 expression in TLR4‐silenced and non‐silenced MSC. A representative experiment is depicted. Statistical analysis was performed using unpaired t‐test, and values are represented as mean ± SEM, three biological replicates.

Source data are available online for this figure.