FIGURE 1:

Point mutations engineered into the MCAK/Kif2C motor domain and analyzed manually exhibit altered depolymerizing activity. (A) The sequence of the MCAK/Kif2C annotated for structural elements and colored to indicate residues exhibiting high identity in the kinesin superfamily (blue), high conservation in the kinesin superfamily (green), high identity in the kinesin-13 family (red), and high conservation in the kinesin-13 family (purple). Mutations to alanine (“A”) or other residues are also indicated. (B) Proportion of MT-depolymerizing activity normalized to GFP-WT-MCAK/Kif2C. N = number of cells individually measured. Violin plots show the median activity (red line).