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. 2020 Mar 19;31(7):580–588. doi: 10.1091/mbc.E19-09-0503

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© 2020 Wagenbach et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — CellProfiler improves the workflow for the analysis of MCAK/Kif2C cancer mutations. (A) A field of cells transfected with GFP-WT-MCAK/Kif2C. GFP, green; MTs, red; DNA, blue. (B) CellProfiler pipeline to analyze cytoplasmic tubulin levels in transfected cells. (C) Missense mutations and truncations mapped to full-length MCAK/Kif2C (cBioPortal). (D) Missense mutations that we engineered were biased toward hot spots (defined as more than two patients). (E) Proportion of MT-depolymerizing activity measured in engineered cancer mutations. N = successive 20× fields of cells containing between one and three transfected cells. Violin plots show the median activity (red line). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.