. 2020 Mar 19;31(7):709–723. doi: 10.1091/mbc.E19-10-0559

TABLE 1:

Parameters of diffusion properties for membrane probes shown in Figure 1B are determined by fitting very large ImFCS data sets with Eq. 3 (D values) or Eq. 5 (τ₀ and Slope = 1/D_eff).

Probes	Membrane association	Treatment	D_fast (μm²/s)^a	D_slow (μm²/s)^a	F_slow	<D_CDF> (μm²/s)^b	D_a_v (μm²/s)^c	τ_0,av (s)^c	Slope_av (s/μm²)^c	N_Px/N_sv (no. of cells)^d
EGFP-GG	Lipid probe, Inner leaflet, Ld-preferring	No	0.66 ± 0.19	0.61 ± 0.09	0.41	0.64 ± 0.12	0.64 ± 0.002	0.19 ± 0.005	1.18 ± 0.01	10,527/648 (18)
	Lipid probe, Inner leaflet, Ld-preferring	CytoD	0.71 ± 0.24	0.66 ± 0.13	0.76	0.67 ± 0.15	0.67 ± 0.002	0.18 ± 0.005	1.14 ± 0.01	9366/539 (16)
PM-EGFP	Lipid probe, Inner leaflet, Lo-preferring	No	0.67 ± 0.24	0.58 ± 0.13	0.63	0.61 ± 0.12	0.61± 0.002	0.27 ± 0.006	1.05 ± 0.02	9375/540 (15)
	Lipid probe, Inner leaflet, Lo-preferring	CytoD	0.74 ± 0.19	0.56 ± 0.13	0.84	0.59 ± 0.11	0.59 ± 0.001	0.27 ± 0.002	1.15 ± 0.01	12,432/720 (20)
YFP-GL-GPI	Lipid probe, Outer leaflet, Lo-preferring	No	0.40 ± 0.10	0.30 ± 0.06	0.72	0.33 ± 0.05	0.33 ± 0.001	0.72 ± 0.009	1.56 ± 0.02	12,892/719 (21)
	Lipid probe, Outer leaflet, Lo-preferring	CytoD	0.36 ± 0.08	0.28 ± 0.05	0.76	0.30 ± 0.04	0.30 ± 0.001	0.73 ± 0.01	1.84 ± 0.02	11,074/661 (19)
Lyn-EGFP	Lipid-anchored probe, Inner leaflet, Lo-preferring	No	0.57 ± 0.15	0.45 ± 0.11	0.69	0.49 ± 0.09	0.49 ± 0.001	0.44 ± 0.009	1.13 ± 0.02	10,000/575 (16)
	Lipid-anchored probe, Inner leaflet, Lo-preferring	CytoD	0.62 ± 0.17	0.44 ± 0.10	0.80	0.48 ± 0.09	0.48 ± 0.001	0.30 ± 0.006	1.55 ± 0.02	12,904/753 (21)
AF488-IgE-FcεRI	TM probe, 7 TMD	No	0.22 ± 0.07	0.14 ± 0.04	0.69	0.16 ± 0.03	0.17 ± 0.001	1.63 ± 0.01	2.56 ± 0.04	27,707/1644 (46)
		CytoD	0.23 ± 0.09	0.13 ± 0.03	0.63	0.17 ± 0.04	0.17 ± 0.001	1.93 ± 0.03	2.39 ± 0.07	10,624/558 (17)
YFP-GL-GT46	TM probe, 1 TMD	No	0.32 ± 0.09	0.21 ± 0.04	0.76	0.24 ± 0.05	0.24 ± 0.001	1.09 ± 0.01	1.99 ± 0.04	6475/385 (12)
		CytoD	—
EGFR-EGFP	TM probe, 1 TMD	No	0.37 ± 0.11	0.24 ± 0.05	0.76	0.27 ± 0.04	0.27 ± 0.001	1.16 ± 0.02	1.23 ± 0.04	6440/384 (12)
		CytoD	—
AcGFP-Orai1	TM probe, 4 TMD	No	0.18 ± 0.05	0.13 ± 0.03	0.70	0.15 ± 0.03	0.15 ± 0.001	2.56 ± 0.03	0.99 ± 0.04	6875/334 (11)
		CytoD	—

TMD = transmembrane domain.

^a± values are standard deviations of the fitted Gaussian distributions (Eq. 3).

^b± values are weighted standard deviations calculated as the propagation of the errors of D_fast and D_slow.

^c± values are standard error of the mean (SEM) for the arithmetic average.

^dN_Px = number of Px units at which D values are determined and N_sv = number of Sv units at which τ₀ and slope values are determined.