FIGURE 1:

Heat dissipation in the early zebrafish embryo. (A) Schematic of an ITC experiment. Thirty embryos from a single pair of parents were collected and staged at the two-cell stage. Following staging, the embryonic heat dissipation rate during development was measured using ITC. (B) The time course of heat dissipation for nine experiments at 23.5°C (gray lines) together with the mean (black line). Time zero corresponds to the beginning of cleavage at the two-cell stage. Positive heat dissipation corresponds to heat transfer from the embryo to the surroundings. (C) The time course of heat dissipation for nine experiments at 28.5°C (gray lines) together with the mean (black line). (D) The time course of heat dissipation for six experiments at 33.5°C (gray lines) together with the mean (black line).