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. Author manuscript; available in PMC: 2020 May 6.
Published in final edited form as: Cell Calcium. 2020 Feb 28;87:102187. doi: 10.1016/j.ceca.2020.102187

Figure 5. TRPM7 knock-down does not decrease SOCE in the ameloblast cell line LS8 cells.

Figure 5.

(A-C) mRNA expression of Trpm7, Orai1 and Orai2 and in Trpm7 knock-down (siTrpm7) LS8 cells by RT-PCR. (D) Representative original tracings of [Ca2+]cyt transients in LS8 cells measured after pre-incubation with thapsigargin (20 minutes, 1 μM), followed by perfusion with a Ca2+-free Ringer’s solution (120 seconds) before the simultaneous re-addition of 2 mM extracellular Ca2+ to stimulate SOCE in the presence or absence of naltriben (NAL,100 μM). TRPM7 activation elicited by NAL (100 μM) does not potentiate the delta SOCE peak (E), SOCE slope (F) or A.U.C. (G) in siTrpm7 LS8 cells. Data represent the mean ± SEM of minimum three independent experiments indicated in the histogram bars. Data analyzed by two-tailed unpaired Student’s t-test at *P < 0.05; **P < 0.01; ***P < 0.001; n.s., non-significant.