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. 2020 Mar 16;21(5):e48780. doi: 10.15252/embr.201948780

Figure 5. Targeting CSF‐1 in mesenchymal‐like PDAC cells abolishes PSC deactivation.

Figure 5

  1. Brightfield images of PS‐1 cells after 72 h of treatment with CM of control (CM of PS‐1s) or mesenchymal PDAC cell lines supplemented with anti‐CSF‐1. Scale bar represents 100 μm.
  2. Gene expression levels of COL1A1 in PS‐1 cells after treatment indicated in panel A using qPCR. Graph shows n = 3 technical replicates of an example experiment that was performed three times.
  3. Brightfield images of PS‐1 cells after 72 h of treatment with PANC‐1 CM of pLKOctrl or shCSF1 (#560–562). Scale bar represents 100 μm.
  4. Correlation graph of CSF1 expression in PANC‐1 pLKOctrl or shCSF1 (#560–562) and how these various CM affect COL1A1 expression in PS‐1 cells using qPCR, and values were normalized for RPLPO expression. n = 3 biological replicates for all individual experiments, error bars indicated SD. R 2 and P‐value were analyzed with linear regression.
  5. Brightfield images of PS‐1 cells after 72 h of treatment with CM of Capan‐2 cells with control vector or Capan‐2 cells overexpressing CSF1. Scale bar represents 100 μm.
  6. Gene expression level of CSF1 in Capan‐2 cells after transduction with control vector or overexpressing CSF1. n = 3 technical replicates per group.
  7. Gene expression levels of COL1A1 in PS‐1 cells after treatment indicated in panel E using qPCR. Graph shows n = 3 technical replicates of an example experiment that was performed three times.
Data information: (B, F, G), data are represented as mean ± SD, Student's t‐test.