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. 2020 Mar 29;21(5):e49124. doi: 10.15252/embr.201949124

Figure EV2. Activation of GABAB receptors inhibits Piezo1 currents in heterologous systems via Gβγ.

Figure EV2

  • A, B
    Representative MA currents recorded in whole‐cell patch‐clamp experiments at −60 mV in HEK293 cells transiently transfected with Piezo1 + GABAB1‐YFP + GABAB2 in response to mechanical stimulation with a blunt glass probe displaced 2–5.6 μm in 0.4‐μm increments every 15 s before (black) and after (red) exposure to 25 μM baclofen.
  • C
    Quantification of MA current inactivation rate (Tau) before and after treating the cells with baclofen, paired t‐test (n = 8). Data are shown as mean ± SEM and scatter plots.
  • D–F
    Quantification of normalized MA currents of HEK293 cells transfected with Piezo1 + GABAB1‐YFP + GABAB2 (D, n = 13), with Piezo1‐IRES‐EGFP (E, n = 9) and Piezo1 + GABAB1‐YFP + GABAB2 + βARKct (F, n = 8) before and after application of 25 μM baclofen (*P < 0.05, **P < 0.01, repeated‐measures ANOVA with Student's t‐test no corrections). Data are shown as mean ± SEM.