Figure 4. PI3K and MAPK participate in the potentiation of Piezo2 currents after activation of GABA_B receptors.

• A–C
  Quantification of normalized MA Piezo2 currents in HEK293 cells transiently transfected with Piezo2 + GABA_B1 + GABA_B2 + GFP (A, n = 12), treated with the PI3K inhibitor wortmannin 50 nM (B, n = 10) or treated with the MAPK inhibitor U0126 10 μM (C, n = 7). *P < 0.05, repeated‐measures ANOVA with Student's t‐test no corrections. Data shown as mean ± SEM. Cells were treated with wortmannin or U0126 for 30 min before the experiments and were present throughout the measurements.
• D
  Putative pathway of regulation of Piezo2 currents by Gi‐coupled receptors; activation of Gi‐coupled receptors promotes the release of Gβγ activating PI3K and MAPK kinase, thus indirectly potentiating Piezo2 currents.