Figure EV5. Forced expression of ECE2 causes neuronal mislocalisation in the developing mouse cortex.
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A, B(A) Example images of CTRL‐electroporated mouse brains E13–16 and (B) example images of mouse brains at 3 dpe after forced expression of ECE2 with IHC for Sox2+ neural progenitors and Ctip2+ deep layer neurons reveal ECE2’s role in neuronal positioning.
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CProtein interaction analysis 52 shows network with ECE2 (red circle) and three interacting proteins that were identified as downregulated upon KO (green squares), including the known PH gene FLNA.
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DNetwork analysis 52 reveals that downregulated proteins in ECE2 KO COs are part of a tightly interconnected PPI network with ECM proteins as the biggest subnetwork.