Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 29;11:476. doi: 10.3389/fpls.2020.00476

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Werner, Gaffar, Schuemann, Biedenkopf and Koch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Direct comparison of long (manual) and short (tool) constructs. Relative expression of the respective fungal DCLs and AGOs 5 dpi on dsRNA-sprayed leaves is grouped by the target gene. The expression was measured via the ΔΔ-Ct method in which the expression of the respective AGOs and DCLs was normalized against the fungal reference gene EF1α (translation elongation-factor 1 α) and β-tubulin, and this Δ-Ct value was then normalized against the Δ-Ct of the GFP control. The asterisks indicate a significant expression of the sprayed leaves in comparison to the mock-treated TE controls. Bars represent mean values ± SE of the four independent experiments.