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. 2020 Mar 18;21(5):e48843. doi: 10.15252/embr.201948843

Figure 1. Mts is required for dendrite pruning in ddaC neurons.

Figure 1

  • A
    A Schematic representation of dendrite pruning in ddaC neurons.
  • B–F
    Live confocal images of ddaC neurons expressing UAS‐mCD8‐GFP driven by ppk‐Gal4 at WP and 16 h APF stages. Dendrites of ctrl RNAi (B), mts RNAi #1 (C), ctrl MARCM (D), mts 299 MARCM (E), and mts 299 rescue (F) ddaC neurons at WP and 16 h APF stages. Red arrowheads point to the ddaC somas.
  • G, H
    UAS‐mts‐dn ddaC neurons from animals in RU486‐induced condition (H) driven by GeneSwitch‐Gal4‐2295 exhibited normal arbors at WP stage and severe dendrite pruning defects at 16 h APF, compared to those in a non‐induced condition (G). Red arrowheads point to the ddaC somas.
  • I–K
    Quantification of number of primary and secondary dendrites attached to soma and percentage of severing defects at 16 h APF.
Data information: In (I–K), data are presented as mean ± SEM from three independent experiments. One‐way ANOVA with Bonferroni test (I) and two‐tailed Student's t‐test (K) were applied to determine statistical significance. ns, not significant; ***P < 0.001. The number of neurons (n) examined in each group is shown on the bars. Scale bars in (B–H) represent 50 μm.Source data are available online for this figure.