Table 1.

Selected commercial nucleic acid-based point-of-care tests (ordered by time to result).

POCT commercial name	Method/Time to result (min)	Detection of new and emerging respiratory viruses: Sensitivity (%)/Specificity(%)
		HBoV	SARS-CoV	SARS-CoV-2	MERS-CoV	HCoV-HKU1	HCoV-NL63	KIPyV, WUPyV	HMPV	RV-C	Emerging IAV
Cepheid Xpert® Xpress Flu/RSVb,c1	rRT-PCR/20-30	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	H1N1pdm09, H3N2, AIV (H5N2, H5N8) 97.8/100
Mesabiotech™ Accula™ Influenza A&B and RSVa2	RT-PCR/30	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	H1N1pdm09, H3N2 97/94
Sekisui Diagnostics Silaris™ Influenza A&B Test3	PCR/30	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	H1N1pdm09, H3N2 97/94
Cepheid Xpert® Flu/RSV XCb,c	rRT-PCR/40-63	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	H1N1pdm09, H3N2, AIV (H5N2, H5N8) •
(Cepheid Xpert® Xpress SARS-CoV-2, 2020)e	rRT-PCR/ 45	N/A	N/A	•	N/A	N/A	N/A	N/A	N/A	N/A	N/A
BioMérieuxBioFire® FilmArray® Respiratory Panel 2 plus4	Endpoint melt curve analysis/45	N/A	N/A	N/A	•	95.8/99.8	95.8/100	N/A	94.6/99.2	N/A	H1N1pdm09, H3N2 88.9-100/99.6-100
Quidel® Solana® Respiratory Viral Panel Influenza A&B and RSV & HMPVa5	RT-HDA/45	N/A	N/A	N/A	N/A	N/A	N/A	N/A	•	N/A	Strains not specified •
DiaSorin Simplexa™ Flu A/B & RSV Direct Kit6	RT-PCR/60	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	H1N1pdm09, H3N2, >20 AIVs, 2 swine influenza strains 97/97.9
Cepheid Xpert® Flub	rRT-PCR/75	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	H1N1pdm09 97.1-100/99.6
Luminex Verigene® RP Flex Test7	RT-PCR/ <120	N/A	N/A	N/A	N/A	N/A	N/A	N/A	•	N/A	H1, H3 •
GenMark Dx® ePlex® Respiratory Paneld	PCR/ <120	N/A	N/A	N/A	N/A	•	•	N/A	N/A	N/A	H1N1pdm09, H3N2 •
(GenMark Dx ePlex® SARS-CoV-2 Test, 2020) Teste	rRT-PCR/ <120	N/A	N/A	•	N/A	N/A	N/A	N/A	N/A	N/A	N/A
(QIAGEN, 2019) Panel v2	rRT-PCR/120	100/99.5	N/A	N/A	N/A	100/100	91.7/100	N/A	100/100	N/A	H1N1pdm09, H3N2 91.7-100/100

¹Cepheid Xpert Flu. Product Page. Available online at: https://www.cepheid.com/en/cepheid-solutions/clinical-ivd-tests/critical-infectious-diseases/xpert-flu (accessed September 13, 2019).

²Mesabiotech™ Accula™ Flu A/FluB Test Package Insert. Manual. Available online at: https://static1.squarespace.com/static/5ca44a0a7eb88c46af449a53/t/5cddacb3f9689300010d93c3/1558031549318/LBL-60010+Accula+Flu+A+Flu+B+Package+Insert+EU.pdf (accessed November 10, 2019).

³Sekisui Diagnostics Silaris™ Influenza A&B Test. Manual. 481. Available online at: https://sekisuidiagnostics.com/product-documents/60012-d_v1.5_silaris_ifu.pdf (accessed November 10, 2019).

⁴Biomerieux BIOFIRE^® FILMARRAY^® Panels. Product Page. Available online at: https://www.biomerieux-diagnostics.com/filmarrayr-respiratory-panel (accessed September 13, 2019).

⁵Quidel^® Solana Respiratory Viral Panel. Product Page. Available online at: https://www.quidel.com/molecular-diagnostics/respiratory-viral-panel (accessed November 10, 2019).

⁶DiaSorin Simplexa^® Flu A/B and RSV Direct Kit. Product Page. Available online at: https://molecular.diasorin.com/us/kit/simplexa-flu-ab-rsv-direct-kit/ (accessed November 10, 2019).

⁷Luminex Verigene® Respiratory Pathogens Flex Test. Product Page. Available online at: https://www.luminexcorp.com/respiratory-pathogens-flex-test/ (accessed September 13, 2019).

N/A, Virus not included in this assay; • Virus included, but specificity/sensitivity not available; RT-HAD, Reverse transcriptase helicase-dependent amplification; RT-PCR, Reverse transcriptase polymerase chain reaction; rRT-PCR, real-time RT-PCR; HBoV, Human bocavirus; (H)CoV, (Human) coronavirus; KI/WUPyV, KI/WU polyomavirus; HMPV, Human metapneumovirus; RV-C, Human rhinovirus type C; IAV, Influenza A virus; IBV, Influenza B virus; H1N1pdm09, Influenza A H1N1 pandemic 2009; AIV, Avian influenza virus; RSV, Respiratory Syncytial virus.

Please refer to Supplementary Table 1 for detailed reliability parameters.

^aTwo different kits for Influenza (A, B) and RSV;

^bPerformance reviewed in Basile et al. (2018);

^cDescribed in Loeffelholz et al. (2019);

^dPerformance tested by Babady et al. (2018);

^eFor use under the Emergency Use Authorization (EUA) only.